fluoroshield tm with dapi  (Beyotime)

Journal: Antioxidants

Article Title: Protective Effects of Beta-3 Adrenoceptor Agonism on Mucosal Integrity in Hyperoxia-Induced Ileal Alterations

doi: 10.3390/antiox13070863

Figure Lengend Snippet: ZO-1 immunolabelling in the ileal mucosa. ( a – e ) Representative images of ZO-1 expression (green). Nuclei are counterstained with DAPI (blue). ( f ) Densitometric analysis of ZO-1-positive area. Exposure to hyperoxia significantly decreased ZO-1 expression compared to the normoxic controls ( a , c , f ). The treatment with BRL37344 at 3 mg/kg, but not at 1 mg/kg, had a protective effect ( c – f ). No significant changes were observed in the normoxia-exposed pups, treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§§ p < 0.001 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 50 µm.

Article Snippet: The specimens were washed with PBS and mounted in DAPI anti-fade aqueous medium (Fluoroshield TM with DAPI, Thermo Fisher Scientific).

Techniques: Expressing

Journal: Antioxidants

Article Title: Protective Effects of Beta-3 Adrenoceptor Agonism on Mucosal Integrity in Hyperoxia-Induced Ileal Alterations

doi: 10.3390/antiox13070863

Figure Lengend Snippet: E-cadherin immunolabelling in the ileal mucosa. ( a – e ) Representative images of E-cadherin expression (green; nuclei are counterstained blue with DAPI). ( f ) Densitometric analysis of E-cadherin-positive area. Exposure to hyperoxia significantly decreased E-cadherin expression compared to the normoxic controls ( a , c , f ). The administration of BRL37344 had a partially protective effect ( c – f ). No changes can be observed in the normoxic pups treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °° p < 0.01 and °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§ p < 0.01 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 25 µm.

Article Snippet: The specimens were washed with PBS and mounted in DAPI anti-fade aqueous medium (Fluoroshield TM with DAPI, Thermo Fisher Scientific).

Techniques: Expressing

Journal: Antioxidants

Article Title: Protective Effects of Beta-3 Adrenoceptor Agonism on Mucosal Integrity in Hyperoxia-Induced Ileal Alterations

doi: 10.3390/antiox13070863

Figure Lengend Snippet: ZO-1 immunolabelling in the ileal mucosa. ( a – e ) Representative images of ZO-1 expression (green). Nuclei are counterstained with DAPI (blue). ( f ) Densitometric analysis of ZO-1-positive area. Exposure to hyperoxia significantly decreased ZO-1 expression compared to the normoxic controls ( a , c , f ). The treatment with BRL37344 at 3 mg/kg, but not at 1 mg/kg, had a protective effect ( c – f ). No significant changes were observed in the normoxia-exposed pups, treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§§ p < 0.001 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 50 µm.

Article Snippet: After the final washing step with PBS, the strips were transferred into a droplet of the anti-fade DAPI mounting medium (Fluoroshield TM with DAPI, Thermo Fisher Scientific, Waltham, MA, USA) dripped onto the microscope slide and oriented on their side to visualize the villus axis [ ].

Techniques: Expressing

Journal: Antioxidants

Article Title: Protective Effects of Beta-3 Adrenoceptor Agonism on Mucosal Integrity in Hyperoxia-Induced Ileal Alterations

doi: 10.3390/antiox13070863

Figure Lengend Snippet: E-cadherin immunolabelling in the ileal mucosa. ( a – e ) Representative images of E-cadherin expression (green; nuclei are counterstained blue with DAPI). ( f ) Densitometric analysis of E-cadherin-positive area. Exposure to hyperoxia significantly decreased E-cadherin expression compared to the normoxic controls ( a , c , f ). The administration of BRL37344 had a partially protective effect ( c – f ). No changes can be observed in the normoxic pups treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °° p < 0.01 and °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§ p < 0.01 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 25 µm.

Article Snippet: After the final washing step with PBS, the strips were transferred into a droplet of the anti-fade DAPI mounting medium (Fluoroshield TM with DAPI, Thermo Fisher Scientific, Waltham, MA, USA) dripped onto the microscope slide and oriented on their side to visualize the villus axis [ ].

Techniques: Expressing